MipTec Proceedings: Transcriptional Assay Screens Using Renilla Luciferase as an Internal Control
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منابع مشابه
PAST PROJECTS Mechanical control of Renilla Luciferase
Enzymes are deformable molecules: the molecular structure can exist in essentially a continuum of different conformations. These are accessible by applying mechanical stress to the molecule. Mechanics then provides a general molecular mechanism to modulate enzymatic reactions, by applying mechanical stresses which deform the enzyme. There are approximately 10 known different enzymatic reactions...
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We have developed a novel system designated the luciferase assay for protein detection (LAPD) to study protein-protein interactions. This method involves two protein fusions, a soluble reporter fusion and a fusion for immobilizing the target protein. The soluble reporter is an N-terminal Renilla luciferase fusion protein that exhibits high Renilla luciferase activity. Crude cleared lysates from...
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1.Holodniy, M., S. Kim, D. Katzenstein, M. Konrad, E. Groves, and T.C. Merigan. 1991. Inhibition of human immunodeficiency virus gene amplification by heparin. J. Clin. Microbiol. 29:676-679. 2.Izraeli, S., C. Pfleiderer, and T. Lion. 1991. Detection of gene expression by PCR amplification of RNA derived from frozen heparinized whole blood. Nucleic Acids Res. 19:6051. 3.Imai, H., O. Yamada, S. ...
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The Renilla bioluminescent system in vivo is comprised of three proteins--the luciferase, green-fluorescent protein, and coelenterazine-binding protein (CBP), previously called luciferin-binding protein (LBP). This work reports the cloning of the full-size cDNA encoding CBP from soft coral Renilla muelleri, its overexpression and properties of the recombinant protein. The apo-CBP was quantitati...
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ژورنال
عنوان ژورنال: JALA: Journal of the Association for Laboratory Automation
سال: 1998
ISSN: 1535-5535
DOI: 10.1177/221106829800300406